GHK-Cu (Copper Peptide)
฿800.00
- Mechanism: Copper-chelating tripeptide with tissue-repair and collagen-stimulating activity
- Format: Lyophilized powder, 50mg per vial, >99% purity by HPLC
- Included: Vial + Certificate of Analysis (HPLC + mass spectrum)
For research use only. Not for human or veterinary consumption. In-vitro laboratory use only.
Description
Product Specifications
| Peptide | GHK-Cu (Glycyl-L-histidyl-L-lysine copper complex) |
|---|---|
| Also known as | Copper peptide • GHK • GHK-copper |
| CAS Number | 49557-75-7 |
| Molecular Weight | 403.91 Da |
| Quantity per vial | 50mg |
| Format | Lyophilized powder (white to off-white) |
| Purity | >99% by HPLC |
| Peptide content | ≥90% net peptide (adjusted for water and counter-ions) |
| Verification method | External HPLC chromatography + mass spectrometry |
| Certificate of Analysis | Provided digitally with every shipment |
| Reconstitution | Sterile water for injection |
| Storage — lyophilized | −20°C, sealed vial, away from moisture and light |
| Storage — in solution | −80°C; use within 30 days; avoid freeze-thaw cycles |
| Shelf life | 24 months from manufacture date (lyophilized, correct storage) |
| Intended use | In-vitro laboratory research only. Not for human or veterinary use. |
Research Background
GHK-Cu (glycyl-L-histidyl-L-lysine copper complex) is a naturally occurring tripeptide found in human plasma, urine, and saliva. It forms a stable complex with copper(II) ions, which is central to its activity in cellular signaling. First isolated in 1973 by Loren Pickart, GHK-Cu has been studied for its role in wound healing, anti-inflammatory signaling, and extracellular matrix remodeling. In cell culture models it has been shown to stimulate collagen, elastin, and glycosaminoglycan synthesis, and to activate the ubiquitin-proteasome pathway for removal of damaged proteins.
The copper-bound form modulates a wide range of genes. Published microarray studies have catalogued over 4,000 human genes affected, including upregulation of growth-related genes and downregulation of those associated with inflammation and oxidative stress. Research interest has expanded to its effects on keratinocyte proliferation, fibroblast activity, and angiogenesis in in-vitro models.
Research Applications
- Fibroblast proliferation and collagen/elastin synthesis assays in 2D and 3D culture models
- Extracellular matrix remodeling pathway characterization (MMPs, TIMPs, collagen crosslinking)
- Wound-closure and scratch assays using keratinocyte or dermal fibroblast cell lines
- Anti-inflammatory cytokine profiling (IL-1β, TNF-α, IL-6) in macrophage stimulation studies
- Angiogenesis assays (HUVEC tube formation, VEGF expression)
- Gene expression profiling of copper-chelating peptide activity via RNA-seq or microarray
- Ubiquitin-proteasome pathway studies and damaged protein clearance models
Reconstitution Protocol
Allow the vial to reach room temperature before opening. Reconstitute in sterile water for injection at the desired working concentration. Do not vortex; gently swirl or invert until the powder is fully dissolved. For cell-based assays requiring sterility, filter through a 0.22 μm membrane. Aliquot immediately into single-use volumes to prevent repeated freeze-thaw cycling.
Storage and Stability
Store lyophilized vials at −20°C in the original sealed container, protected from moisture and light. Lyophilized material is stable for up to 24 months from the manufacture date shown on the Certificate of Analysis. Reconstituted solutions should be stored at −80°C and used within 30 days. Each freeze-thaw cycle degrades the peptide; aliquoting before freezing is strongly recommended.
What’s Included
- 1 × GHK-Cu lyophilized vial — 50mg
- Independent Certificate of Analysis (HPLC chromatogram + mass spectrum + batch data)
- Batch number and expiry date printed on vial label
- Discreet external packaging with no product name or branding on the exterior
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